Test

Urine Routine & Microscopy

Urine routine and microscopy test: what it checks (pH, protein, sugar, pus cells), normal values, preparation, and how to book online.

Key Takeaways

What you need to know at a glance

Urine R/M is a simple, non-invasive test that screens for UTIs, kidney disease, and diabetes.
A mid-stream clean-catch sample, ideally first-morning urine, gives the most accurate results.
Pus cells > 5/HPF with positive nitrites strongly suggest a urinary tract infection.
Protein in urine (proteinuria) is an early warning sign of kidney damage, especially in diabetics.
Full Article

What It Measures#

A Urine Routine and Microscopy (Urine R/M) test is a basic, non-invasive screening test that examines the physical, chemical, and microscopic properties of your urine. It includes:

Physical examination: Colour, appearance (clear or turbid), specific gravity.

Chemical analysis (dipstick):

  • pH — acidity or alkalinity of urine.
  • Protein — normally absent; presence suggests kidney damage.
  • Glucose — normally absent; present in uncontrolled diabetes.
  • Ketones — elevated in diabetic ketoacidosis, prolonged fasting, or low-carb diets.
  • Blood (occult) — indicates infection, stones, or glomerular disease.
  • Bilirubin & Urobilinogen — liver or bile-duct issues.
  • Nitrites & Leukocyte Esterase — suggestive of bacterial urinary tract infection (UTI).

Microscopy: Examines the urine sediment for pus cells (WBCs), RBCs, epithelial cells, casts, crystals, and bacteria.

Who Should Get Tested#

  • Anyone with urinary symptoms: burning, frequency, urgency, foul smell, or blood in urine.
  • Patients with suspected UTI — especially women, who are more prone.
  • Diabetics — to screen for proteinuria (early kidney damage) and glycosuria.
  • Pregnant women — routine at every antenatal visit to check for pre-eclampsia.
  • People with kidney stones — to identify crystal types.
  • As part of a routine health check-up.

How to Prepare#

  • Collect a mid-stream, clean-catch urine sample in a sterile container.
  • First-morning sample is preferred for the most concentrated results.
  • Clean the genital area before collection to avoid contamination.
  • Deliver the sample to the lab within 1–2 hours of collection (or refrigerate).

Understanding Your Results#

| Parameter | Normal | Abnormal Suggests | |-----------|--------|-------------------| | Colour | Pale to dark yellow | Red/brown = blood; dark yellow = dehydration | | pH | 4.5–8.0 | Persistent alkaline = UTI, renal tubular acidosis | | Protein | Nil or trace | Kidney disease, pre-eclampsia | | Glucose | Nil | Uncontrolled diabetes | | Pus Cells | 0–5 /HPF | > 5 = UTI or inflammation | | RBCs | 0–2 /HPF | Stones, infection, glomerulonephritis | | Casts | Nil | Renal tubular damage, nephrotic syndrome | | Crystals | Nil or few | Calcium oxalate, uric acid (stone risk) |

A urine R/M showing pus cells > 5/HPF with nitrites is highly suggestive of a bacterial UTI and usually requires a urine culture and sensitivity test for antibiotic selection.

Related Tests#

Booking & Home Collection#

Book a urine routine test on PingMeDoc. Home collection kits with sterile containers are provided. Results are typically available within 6–12 hours.

Frequently Asked Questions

5 questions answered by our medical team

1
What is a mid-stream clean-catch sample?

Start urinating into the toilet, then collect the middle portion of the stream in the sterile container. This avoids contamination from bacteria around the urethral opening.

2
Can urine test detect pregnancy?

A standard urine R/M does not test for pregnancy. A separate urine pregnancy test (UPT) detects the hormone hCG and is a different test.

3
Why is my urine dark yellow?

The most common reason is dehydration — drink more water. Other causes include certain medications (like rifampicin, which turns urine orange) and B-complex vitamins (bright yellow).

4
What does protein in urine mean?

Small amounts of protein can appear after intense exercise or fever and are usually harmless. Persistent proteinuria indicates kidney damage and requires further evaluation with a kidney function test and urine albumin-to-creatinine ratio (ACR).

5
How quickly should I deliver the sample to the lab?

Ideally within 1–2 hours of collection. Delays can cause bacterial overgrowth, alkaline pH shift, and disintegration of casts and cells, leading to inaccurate results.

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References & Sources

2 cited sources

  1. 1

    Urinalysis

    Mayo Clinic2024View source
  2. 2

    Urine Routine and Microscopy — Standardisation in Indian Labs

    Indian Journal of Clinical Biochemistry2020

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