The Vitamin D Total (25-OH) test measures 25-hydroxyvitamin D — the storage form of vitamin D and the most reliable marker of your body's vitamin D status from both sunlight and diet. Vitamin D is essential for calcium absorption, strong bones, muscle function and a healthy immune system. Deficiency is very common in India despite abundant sunshine, owing to limited sun exposure, indoor lifestyles and darker skin tones.
Clinical use cases
Why doctors order it
Bone and immunity assessment
Vitamin D deficiency
Osteomalacia
Rickets
Osteoporosis
Hypocalcaemia
About this test
The Vitamin D Total (25-OH) test measures 25-hydroxyvitamin D — the storage form of vitamin D and the most reliable marker of your body's vitamin D status from both sunlight and diet. Vitamin D is essential for calcium absorption, strong bones, muscle function and a healthy immune system. Deficiency is very common in India despite abundant sunshine, owing to limited sun exposure, indoor lifestyles and darker skin tones.
Clinical significance
Low 25-OH vitamin D impairs calcium absorption and, over time, softens and weakens bones — rickets in children and osteomalacia or osteoporosis in adults — raising fracture risk. Deficiency is also linked to muscle weakness, fatigue, low mood and reduced immunity. Very high levels, usually from over-supplementation, can cause vitamin D toxicity with raised blood calcium.
Get tested if you have these symptoms
Bone pain or frequent fractures
Muscle weakness, aches or cramps
Tingling, pins-and-needles or numbness in the hands and feet
Persistent tiredness or fatigue
Low mood or low energy
Frequent infections or low immunity
Hair loss
What this test detects
Vitamin D deficiency
Osteomalacia
Rickets
Osteoporosis
Hypocalcaemia
Secondary hyperparathyroidism
Normal ranges
Deficiency: < 10 ng/mL
Insufficiency: 10–30 ng/mL
Sufficiency: 30–100 ng/mL
Toxicity: > 100 ng/mL
Reference ranges vary by lab and patient demographic. Interpret with a clinician.
Sample & preparation
Sample type
Blood (Serum)
Fasting
Not required
Report TAT
Same day / 24 hours
Home collection
Available
How to prepare
1No fasting required
2Tell the clinician if you take vitamin D or calcium supplements — recent doses can raise levels
What is the Vitamin D - Total (25-OH Vit D) - Serum?
The Vitamin D Total (25-OH) test measures 25-hydroxyvitamin D — the storage form of vitamin D and the most reliable marker of your body's vitamin D status from both sunlight and diet. Vitamin D is essential for calcium absorption, strong bones, muscle function and a healthy immune system. Deficiency is very common in India despite abundant sunshine, owing to limited sun exposure, indoor lifestyles and darker skin tones.
What conditions does the Vitamin D - Total (25-OH Vit D) - Serum detect?
The Vitamin D - Total (25-OH Vit D) - Serum helps detect and monitor conditions including Vitamin D deficiency, Osteomalacia, Rickets, Osteoporosis, Hypocalcaemia, Secondary hyperparathyroidism.
How should I prepare for the Vitamin D - Total (25-OH Vit D) - Serum?
No fasting required | Tell the clinician if you take vitamin D or calcium supplements — recent doses can raise levels | Continue routine medicines unless advised otherwise | Stay normally hydrated
What is the normal range for the Vitamin D - Total (25-OH Vit D) - Serum?
Deficiency: < 10 ng/mL
Insufficiency: 10–30 ng/mL
Sufficiency: 30–100 ng/mL
Toxicity: > 100 ng/mL Note: reference ranges may vary by laboratory and should be interpreted by your doctor in the context of your overall health.
How much does the Vitamin D - Total (25-OH Vit D) - Serum cost?
The Vitamin D - Total (25-OH Vit D) - Serum is available at ₹2299 on PingMeDoc™ with free home sample collection. Prices are inclusive of sample collection charges.
Why is vitamin D deficiency so common in India?
Despite plenty of sunshine, most people in India get little direct sun because of indoor work, covered clothing, sunscreen and darker skin tones. As a result, vitamin D deficiency is seen across all ages and regions.
Can vitamin D deficiency cause tingling or pins and needles?
Yes. Low vitamin D lowers blood calcium, which can cause tingling, pins-and-needles or numbness in the hands and feet, along with muscle cramps and weakness. A 25-OH vitamin D test helps confirm whether low vitamin D is the cause.
How much does the vitamin D test cost and is home collection available?
The Vitamin D (25-OH) test is ₹2299 on PingMeDoc with free home sample collection. A trained phlebotomist collects the sample at your home and your digital report is ready the same day or within 24 hours.
How is vitamin D deficiency treated?
Treatment is usually vitamin D supplementation — often weekly high-dose sachets or capsules — along with sensible sun exposure and dietary changes. Levels are typically rechecked after 8–12 weeks.
Does the Vitamin D - Total (25-OH Vit D) - Serum test require fasting?
No fasting is required for the Vitamin D - Total (25-OH Vit D) - Serum test. You can eat and drink normally before sample collection.
How much does the Vitamin D - Total (25-OH Vit D) - Serum test cost at PingMeDoc?
The Vitamin D - Total (25-OH Vit D) - Serum test costs ₹2299 at PingMeDoc. Free home sample collection is included. NABL-accredited lab processing. Reports delivered digitally in same day / 24 hours.
How long does it take to get Vitamin D - Total (25-OH Vit D) - Serum test results?
Vitamin D - Total (25-OH Vit D) - Serum test results are typically available within same day / 24 hours after sample collection. You can view and download your digital report from your PingMeDoc dashboard.
What type of sample is needed for the Vitamin D - Total (25-OH Vit D) - Serum test?
The Vitamin D - Total (25-OH Vit D) - Serum test requires blood (serum). A trained phlebotomist will collect the sample at your home at your preferred time slot.
Is the Vitamin D - Total (25-OH Vit D) - Serum test NABL accredited?
Yes. All lab tests on PingMeDoc, including the Vitamin D - Total (25-OH Vit D) - Serum test, are processed by NABL-accredited and ISO-certified labs. Reports are reviewed and available on your dashboard.
Is home sample collection free for this test?
Yes. Home sample collection for the Vitamin D - Total (25-OH Vit D) - Serum test is free when booking through PingMeDoc. A phlebotomist visits your home or office at your scheduled time slot.
What conditions does the Vitamin D - Total (25-OH Vit D) - Serum test help screen for?
The Vitamin D Total (25-OH) test measures 25-hydroxyvitamin D — the storage form of vitamin D and the most reliable marker of your body's vitamin D status from both sunlight and diet. Vitamin D is essential for calcium absorption, strong bones, muscle function and a healthy immune system. Deficiency is very common in India despite abundant sunshine, owing to limited sun exposure, indoor lifestyles and darker skin tones. It is commonly used for: Vitamin D deficiency, Bone health assessment, Immunity evaluation, Muscle weakness investigation, Fatigue and low mood screening.
How should I prepare for the Vitamin D - Total (25-OH Vit D) - Serum test?
No fasting required. Tell the clinician if you take vitamin D or calcium supplements — recent doses can raise levels. Continue routine medicines unless advised otherwise. Stay normally hydrated.
Test details are for general information only and not a substitute for medical advice. Reference ranges vary between labs. Always discuss results with a registered medical practitioner before starting, stopping or changing any treatment.
₹2,299
Free home collection
What this test measures
1 parameters in the Vitamin D - Total (25-OH Vit D) - Serum
The Vitamin D - Total (25-OH Vit D) - Serum reports the following parameters. These are this lab’s own reference ranges: the same ones your result is reported against, not a generic table. Adult values are shown; where a range differs by age, the range for your age is applied automatically when the result is released. Always read results with your doctor.
Vitamins
Parameter
Reference range
Unit
Method
LOINC
Vitamin D (25-OH)
30 – 100
ng/mL
Derived from VD2 and VD3 values
62292-8
Understanding your results
What high or low results may mean
For the parameter below, here is what a value outside the reference range may be associated with. These are general, textbook associations: a result outside the range is not a diagnosis on its own.
Your bones and minerals
Minerals and vitamins your body uses to build and maintain bone. These markers reflect what is available in your blood, not the strength of the bone itself.
Parameter
If higher than range
If lower than range
Vitamin D (25-OH)
A very high vitamin D is uncommon and is usually related to high-dose supplements.
A low vitamin D is common and may contribute to bone and muscle symptoms.
Reference ranges vary by laboratory, age, sex and method, and results must be interpreted together and in the context of your health. This information is educational and is not a diagnosis or medical advice, always discuss your report with your doctor.
Vitamin D - Total (25-OH Vit D) - Serum price across India
Same transparent price in every city
The Vitamin D - Total (25-OH Vit D) - Serum is ₹2299 in every city we serve, no city surcharge, no hidden fees, and free home sample collection included. Tap your city to book with a NABL-accredited partner lab.
City
Price
Home collection
Book
Vitamin D - Total (25-OH Vit D) - Serum in Chennai
Uniform, transparent pricing is our policy, you pay the same ₹2299 for the Vitamin D - Total (25-OH Vit D) - Serum whether you book in a metro or a smaller city, with home collection always free.
Vitamin D - Total (25-OH Vit D) - Serum: Quick facts
Sample type
Blood (Serum)
Fasting
1
Report TAT
Same day / 24 hours
Price
₹2299
Home collection
Available across India
Clinical Significance
Low 25-OH vitamin D impairs calcium absorption and
over time
softens and weakens bones — rickets in children and osteomalacia or osteoporosis in adults — raising fracture risk. Deficiency is also linked to muscle weakness
fatigue
low mood and reduced immunity. Very high levels
usually from over-supplementation
can cause vitamin D toxicity with raised blood calcium.
Get the Vitamin D - Total (25-OH Vit D) - Serum if you have these symptoms
Bone pain or frequent fractures
Muscle weakness, aches or cramps
Tingling, pins-and-needles or numbness in the hands and feet
Persistent tiredness or fatigue
Low mood or low energy
Frequent infections or low immunity
Hair loss
Frequently Asked Questions
What is the Vitamin D - Total (25-OH Vit D) - Serum?
The Vitamin D Total (25-OH) test measures 25-hydroxyvitamin D — the storage form of vitamin D and the most reliable marker of your body's vitamin D status from both sunlight and diet. Vitamin D is essential for calcium absorption, strong bones, muscle function and a healthy immune system. Deficiency is very common in India despite abundant sunshine, owing to limited sun exposure, indoor lifestyles and darker skin tones.
What conditions does the Vitamin D - Total (25-OH Vit D) - Serum detect?
The Vitamin D - Total (25-OH Vit D) - Serum helps detect and monitor conditions including Vitamin D deficiency, Osteomalacia, Rickets, Osteoporosis, Hypocalcaemia, Secondary hyperparathyroidism.
How should I prepare for the Vitamin D - Total (25-OH Vit D) - Serum?
No fasting required; Tell the clinician if you take vitamin D or calcium supplements — recent doses can raise levels; Continue routine medicines unless advised otherwise; Stay normally hydrated
What is the normal range for the Vitamin D - Total (25-OH Vit D) - Serum?
Deficiency: < 10 ng/mL
Insufficiency: 10–30 ng/mL
Sufficiency: 30–100 ng/mL
Toxicity: > 100 ng/mL Note: reference ranges may vary by laboratory and should be interpreted by your doctor in the context of your overall health.
How much does the Vitamin D - Total (25-OH Vit D) - Serum cost?
The Vitamin D - Total (25-OH Vit D) - Serum is available at ₹2299 on PingMeDoc with free home sample collection across India. Prices are inclusive of sample collection charges.
